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plpcx retrovirus vector  (TaKaRa)


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    TaKaRa plpcx retrovirus vector
    Plpcx Retrovirus Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plpcx+retrovirus+vector/pmc11104289-56-28-31
    Average 86 stars, based on 1 article reviews
    plpcx retrovirus vector - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: A superficial colon tumor model involving subcutaneous colon translocation and orthotopic transplantation of green fluorescent protein-expressing human colon tumor.
    Article Snippet: The orthotopic transplantation model of human tumor has been demonstrated to be more patient-like animal tumor model.. However, observations of tumor progression and metastasis are limited by the deep location of the colon or limited deep penetration ability of fluorescence through tissue.. The purpose of this study is to establish a superficial orthotopic model to allow easier real-time visualization and more sensitive monitoring of fluorescent orthotopic colon tumor.

    Article Title: Pharmacologic Targeting of Histone H3K27 Acetylation/BRD4-dependent Induction of ALDH1A3 for Early-phase Drug Tolerance of Gastric Cancer
    Article Snippet: .. To establish ALDH1A3-overexpressing cells, the human ALDH1A3 open reading frame sequence was amplified by PCR using cDNA from JSC15-3 cells as a template, and then cloned into a pLPCX retrovirus vector (Takara, RRID:Addgene_44471) using a DNA ligation kit Ver 2.1 (Takara). ..

    Sequencing:

    Article Title: Pharmacologic Targeting of Histone H3K27 Acetylation/BRD4-dependent Induction of ALDH1A3 for Early-phase Drug Tolerance of Gastric Cancer
    Article Snippet: .. To establish ALDH1A3-overexpressing cells, the human ALDH1A3 open reading frame sequence was amplified by PCR using cDNA from JSC15-3 cells as a template, and then cloned into a pLPCX retrovirus vector (Takara, RRID:Addgene_44471) using a DNA ligation kit Ver 2.1 (Takara). ..

    Polymerase Chain Reaction:

    Article Title: Pharmacologic Targeting of Histone H3K27 Acetylation/BRD4-dependent Induction of ALDH1A3 for Early-phase Drug Tolerance of Gastric Cancer
    Article Snippet: .. To establish ALDH1A3-overexpressing cells, the human ALDH1A3 open reading frame sequence was amplified by PCR using cDNA from JSC15-3 cells as a template, and then cloned into a pLPCX retrovirus vector (Takara, RRID:Addgene_44471) using a DNA ligation kit Ver 2.1 (Takara). ..

    Clone Assay:

    Article Title: Pharmacologic Targeting of Histone H3K27 Acetylation/BRD4-dependent Induction of ALDH1A3 for Early-phase Drug Tolerance of Gastric Cancer
    Article Snippet: .. To establish ALDH1A3-overexpressing cells, the human ALDH1A3 open reading frame sequence was amplified by PCR using cDNA from JSC15-3 cells as a template, and then cloned into a pLPCX retrovirus vector (Takara, RRID:Addgene_44471) using a DNA ligation kit Ver 2.1 (Takara). ..

    Plasmid Preparation:

    Article Title: Pharmacologic Targeting of Histone H3K27 Acetylation/BRD4-dependent Induction of ALDH1A3 for Early-phase Drug Tolerance of Gastric Cancer
    Article Snippet: .. To establish ALDH1A3-overexpressing cells, the human ALDH1A3 open reading frame sequence was amplified by PCR using cDNA from JSC15-3 cells as a template, and then cloned into a pLPCX retrovirus vector (Takara, RRID:Addgene_44471) using a DNA ligation kit Ver 2.1 (Takara). ..

    DNA Ligation:

    Article Title: Pharmacologic Targeting of Histone H3K27 Acetylation/BRD4-dependent Induction of ALDH1A3 for Early-phase Drug Tolerance of Gastric Cancer
    Article Snippet: .. To establish ALDH1A3-overexpressing cells, the human ALDH1A3 open reading frame sequence was amplified by PCR using cDNA from JSC15-3 cells as a template, and then cloned into a pLPCX retrovirus vector (Takara, RRID:Addgene_44471) using a DNA ligation kit Ver 2.1 (Takara). ..



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    ΔNp63α promotes proliferation and suppresses terminal differentiation in primary human keratinocytes. (A) APC/C degradation-resistant ΔNp63α and Stxbp4 suppress terminal differentiation. Empty vector (Mock), degradation-resistant ΔNp63α (RL7-ΔNp63α), and Stxbp4 retrovirally infected pHKCs were cultured in 1.5 mM CaCl2 to induce differentiation and were imaged by an LSM 700 laser-scanning confocal microscope (Zeiss). (Scale bars: 50 μm.) (B) <t>Retrovirus-infected</t> pHKCs cultured in high-calcium conditions were harvested at the indicated times, and cell lysates were subjected to immunoblotting with the indicated antibodies. (C) Involucrin mRNA level as in B was determined by RT-qPCR. (D) Stxbp4 (ΔCC-Stxbp4) retrovirally infected pHKCs cultured in high-calcium conditions were harvested at the indicated times. The cell lysates were subjected to immunoblotting with the indicated antibodies. Full-length Stxbp4 is shown in B. (E) Involucrin mRNA levels as in D were determined by RT-qPCR. Stxbp4 mRNA is shown in C. (F) Stxbp4 and APC/C-resistant ΔNp63α-infected pHKCs do not undergo terminal differentiation in 3D organotypic raft cultures. 3D skin equivalents were generated using human pHKCs infected with empty vector (Mock), stable RL7-ΔNp63α–, and Stxbp4-expressing retroviruses. Involucrin (Alexa 594: red) and Loricrin (Alexa 488: green) were detected in <t>pLPCX-infected</t> keratinocytes (Left), and human skin epidermis was used for comparison (Lower Right). (Scale bars: 20 μm.) IF, immunofluorescence.
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    Image Search Results


    ΔNp63α promotes proliferation and suppresses terminal differentiation in primary human keratinocytes. (A) APC/C degradation-resistant ΔNp63α and Stxbp4 suppress terminal differentiation. Empty vector (Mock), degradation-resistant ΔNp63α (RL7-ΔNp63α), and Stxbp4 retrovirally infected pHKCs were cultured in 1.5 mM CaCl2 to induce differentiation and were imaged by an LSM 700 laser-scanning confocal microscope (Zeiss). (Scale bars: 50 μm.) (B) Retrovirus-infected pHKCs cultured in high-calcium conditions were harvested at the indicated times, and cell lysates were subjected to immunoblotting with the indicated antibodies. (C) Involucrin mRNA level as in B was determined by RT-qPCR. (D) Stxbp4 (ΔCC-Stxbp4) retrovirally infected pHKCs cultured in high-calcium conditions were harvested at the indicated times. The cell lysates were subjected to immunoblotting with the indicated antibodies. Full-length Stxbp4 is shown in B. (E) Involucrin mRNA levels as in D were determined by RT-qPCR. Stxbp4 mRNA is shown in C. (F) Stxbp4 and APC/C-resistant ΔNp63α-infected pHKCs do not undergo terminal differentiation in 3D organotypic raft cultures. 3D skin equivalents were generated using human pHKCs infected with empty vector (Mock), stable RL7-ΔNp63α–, and Stxbp4-expressing retroviruses. Involucrin (Alexa 594: red) and Loricrin (Alexa 488: green) were detected in pLPCX-infected keratinocytes (Left), and human skin epidermis was used for comparison (Lower Right). (Scale bars: 20 μm.) IF, immunofluorescence.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: STXBP4 regulates APC/C-mediated p63 turnover and drives squamous cell carcinogenesis

    doi: 10.1073/pnas.1718546115

    Figure Lengend Snippet: ΔNp63α promotes proliferation and suppresses terminal differentiation in primary human keratinocytes. (A) APC/C degradation-resistant ΔNp63α and Stxbp4 suppress terminal differentiation. Empty vector (Mock), degradation-resistant ΔNp63α (RL7-ΔNp63α), and Stxbp4 retrovirally infected pHKCs were cultured in 1.5 mM CaCl2 to induce differentiation and were imaged by an LSM 700 laser-scanning confocal microscope (Zeiss). (Scale bars: 50 μm.) (B) Retrovirus-infected pHKCs cultured in high-calcium conditions were harvested at the indicated times, and cell lysates were subjected to immunoblotting with the indicated antibodies. (C) Involucrin mRNA level as in B was determined by RT-qPCR. (D) Stxbp4 (ΔCC-Stxbp4) retrovirally infected pHKCs cultured in high-calcium conditions were harvested at the indicated times. The cell lysates were subjected to immunoblotting with the indicated antibodies. Full-length Stxbp4 is shown in B. (E) Involucrin mRNA levels as in D were determined by RT-qPCR. Stxbp4 mRNA is shown in C. (F) Stxbp4 and APC/C-resistant ΔNp63α-infected pHKCs do not undergo terminal differentiation in 3D organotypic raft cultures. 3D skin equivalents were generated using human pHKCs infected with empty vector (Mock), stable RL7-ΔNp63α–, and Stxbp4-expressing retroviruses. Involucrin (Alexa 594: red) and Loricrin (Alexa 488: green) were detected in pLPCX-infected keratinocytes (Left), and human skin epidermis was used for comparison (Lower Right). (Scale bars: 20 μm.) IF, immunofluorescence.

    Article Snippet: Plasmids expressing FLAG-HA doubly tagged ΔNp63α, FLAG-tagged wild-type and ubiquitination-resistant (RL7) ΔNp63α, and FLAG-tagged Stxbp4 were cloned into the MSCV or pLPCX retrovirus expression vector (Clontech, Takara Bio).

    Techniques: Plasmid Preparation, Infection, Cell Culture, Microscopy, Western Blot, Quantitative RT-PCR, Generated, Expressing, Immunofluorescence